pml i restriction enzyme Search Results


pml i enzyme  (New England Biolabs)
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New England Biolabs pml i enzyme
Bacterial strains and plasmids used in this work
Pml I Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzyme pml i  (New England Biolabs)
95
New England Biolabs restriction enzyme pml i
Bacterial strains and plasmids used in this work
Restriction Enzyme Pml I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmli restriction enzymes  (Thermo Fisher)
86
Thermo Fisher pmli restriction enzymes
Bacterial strains and plasmids used in this work
Pmli Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmli restriction enzyme  (Thermo Fisher)
90
Thermo Fisher pmli restriction enzyme
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Pmli Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fast digest restriction enzyme pmli  (Thermo Fisher)
86
Thermo Fisher fast digest restriction enzyme pmli
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Fast Digest Restriction Enzyme Pmli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmli restriction endonuclease  (Thermo Fisher)
86
Thermo Fisher pmli restriction endonuclease
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Pmli Restriction Endonuclease, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes pmli  (TFS Inc)
90
TFS Inc restriction enzymes pmli
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Restriction Enzymes Pmli, supplied by TFS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzyme pmli  (New England Biolabs)
95
New England Biolabs restriction enzyme pmli
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Restriction Enzyme Pmli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/restriction enzyme pmli/product/New England Biolabs
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pg2rnai2 (genbank: kt954097, from 452th to 4450th nucleotide, 5′ end flanked with bspeⅰ and 3′ end flanked with pmlⅰ restriction enzyme sites)  (GenScript corporation)
90
GenScript corporation pg2rnai2 (genbank: kt954097, from 452th to 4450th nucleotide, 5′ end flanked with bspeⅰ and 3′ end flanked with pmlⅰ restriction enzyme sites)
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Pg2rnai2 (Genbank: Kt954097, From 452th To 4450th Nucleotide, 5′ End Flanked With Bspeⅰ And 3′ End Flanked With Pmlⅰ Restriction Enzyme Sites), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pg2rnai2 (genbank: kt954097, from 452th to 4450th nucleotide, 5′ end flanked with bspeⅰ and 3′ end flanked with pmlⅰ restriction enzyme sites) - by Bioz Stars, 2026-02
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restriction enzyme eco72i (pmli)  (Thermo Fisher)
90
Thermo Fisher restriction enzyme eco72i (pmli)
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Restriction Enzyme Eco72i (Pmli), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes pmli  (Thermo Fisher)
86
Thermo Fisher restriction enzymes pmli
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Restriction Enzymes Pmli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzyme pml i  (Thermo Fisher)
90
Thermo Fisher restriction enzyme pml i
PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by <t>PmlI</t> <t>(ECO72I</t> Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.
Restriction Enzyme Pml I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Bacterial strains and plasmids used in this work

Journal: Scientific Reports

Article Title: Heterologous viral expression systems in fosmid vectors increase the functional analysis potential of metagenomic libraries

doi: 10.1038/srep01107

Figure Lengend Snippet: Bacterial strains and plasmids used in this work

Article Snippet: The metagenomic library was constructed according to the CopyControl™ Fosmid Library Production kit protocol (Epicentre), except that pMPO579 was used instead of pCC1FOS. pMPO579 was linearised by restriction with the Pml I enzyme (New England Biolabs, isoschizomer of Eco 72I), dephosphorylated with Shrimp Alkaline Phosphatase (USB) and concentrated to 300 mg L −1 with a Centrifugal Filter Device (Microcon, Millipore).

Techniques: Acetylene Reduction Assay, Plasmid Preparation, Clone Assay, Expressing, Derivative Assay

PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by PmlI (ECO72I Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.

Journal:

Article Title: Bartonella koehlerae , a New Cat-Associated Agent of Culture-Negative Human Endocarditis

doi: 10.1128/JCM.42.8.3462-3468.2004

Figure Lengend Snippet: PCR-RFLP assays used to distinguish B. koehlerae infections from those caused by other Bartonella species. Restriction enzyme-digested PCR products of Bartonella species were analyzed by polyacrylamide gel electrophoresis. (A) DNA band patterns obtained by PmlI (ECO72I Fermentas enzyme) digestion of gltA amplification products; (B) DNA band patterns obtained by TaqI digestion of ribC PCR products (amplified with primers BARTON-1 and BARTON-2). (A and B) Lanes 1, DNA of a B. henselae from the American Type Culture Collection; lanes 2, 3, and 4, B. henselae, B. quintana, and B. koehlerae-infected specimens from patients with endocarditis, respectively; lanes 5 and 6, DNA from B. clarridgeiae and B. elizabethae strains from the American Type Culture Collection, respectively; lanes M, molecular size standard (in base pairs). The arrows and numbers indicate sizes (in base pairs) of DNA bands.

Article Snippet: Digestion of the gltA amplicon with the PmlI restriction enzyme ( ECO72I Fermentas enzyme; MBI Fermentas, Vilnius, Lithuania) was carried out in a mixture consisting of 10 to 20 μl of the PCR product, 5 μl of the appropriate enzyme buffer, 2 μl (20 U) of enzyme, and double-distilled water to a final volume of 50 μl.

Techniques: Polyacrylamide Gel Electrophoresis, Amplification, Infection